2 x SYBR Green qPCR Mix

1533-2xSYBR qPCR Mix

制品说明：
本制品本制品是采用SYBR® Green I嵌合荧光法进行Real Time PCR的专用试剂。已经将热启动HotMaster Taq DNA聚合酶、dNTP、特殊稳定剂、优化的反应缓冲液、BSA和SYBR® Green I等试剂预混成一种适合Real Time PCR反应检测用2×Premix Type试剂，具有灵敏度高、特异性强、稳定性好等特点。使用时只需加入模板和引物和水，便可在宽广的定量区域内得到良好的标准曲线，对目的基因进行准确定量检测，重复性好，可信度高。本品采用全新的Hotmaster Taq DNA 聚合酶，该酶不同于一般Hot-start 酶之处在于，一般的Hot-start 酶只在第一步温度升高之前封闭酶的活性，而Hotmaster Taq DNA 聚合酶是利用抑制剂通过温度调节方式封闭Hotmaster Taq DNA聚合酶的底物结合位点，温度低于40℃时，形成非活性的酶-抑制剂复合物，当温度升高至引物特异性的退火温度时，结合平衡向模板-特异性引物复合物形成方向移动，因此最大限度的减少PCR扩增全程中的非特异性扩增产物产生，大大提高了荧光定量PCR反应的精确性。

2 x SYBR Green qPCR Mix provides rapid real-time quantification of RNA targets in SYBR Green fluorescent. The components of 2 x SYBR Green qPCR Mix include Hot-start enzyme named HotMaster Taq DNA Polymerase, dNTP, PCR reaction buffer, BSA and SYBR Green , and so on. The fluorescent dye SYBR Green I in the master mix enables the analysis of many different targets without having to synthesize target specific labeled probes. High specificity and sensitivity in PCR are achieved by the use of the hot-start enzyme HotMaster Taq DNA Polymerase together with a specialized PCR buffer. Unlike other “hot-start” Taq DNA polymerase formulations that block the enzyme activity only in the first high temperature step, HotMaster Taq DNA inhibitors closes the polymerase-substrate binding site by temperature adjustment method. Inactive polymerase-inhibitor complexes are formed at temperatures below 40°C. As the temperature is elevated to the primer-specific annealing temperature, the binding equilibrium is shifted towards complex formation only with target-specific primed template DNA. This prevents the formation of misprimed products and primer–dimers during reaction setup and the first denaturation step, leading to high PCR specificity and accurate quantification.  



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博凌科为 2 x SYBR Green qPCR Mix （153302） 实验结果图

博凌科为 TOYOBO 荧光定量PCR试剂平行对照

博凌科为 ABI Bio-Rad 荧光定量PCR Mix 平行对照

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