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Super 组织细胞microRNA提取试剂盒

1105-Super 组织细胞microRNA提取试剂盒
 
目录号:1105
目录编号 包装单位
110501 50
 
适用范围:
适用于快速提取各种细胞组织miRNA和其它各种小RNA

 产品介绍
近年来对RNA干扰和调节性小RNA的广泛研究迫切需要一种能有效提取15­-30核苷酸左右大小RNA(包括siRNA和miRNA)的试剂盒。但是传统的RNA提取方法如硅胶膜不能有效吸附回收,酚/胍抽提和乙醇沉淀并不能有效沉淀回收微小分子RNA。本试剂盒采用独特的裂解液/β-巯基乙醇迅速裂解细胞和灭活细胞RNA酶,强烈有机抽提去除蛋白和DNA,RNA包括微小分子RNA吸附于离心柱内特殊硅基质膜, 再通过一系列快速的漂洗-离心的步骤,漂洗液将细胞代谢物,蛋白等杂质进一步去除, 最后低盐的洗脱液将纯净RNA从硅基质膜上洗脱。
 
 产品特点:
1.离心吸附柱内硅基质膜全部采用进口世界著名公司特制吸附膜,柱与柱之间吸附量差异极小,可重复性好。克服了国产试剂盒膜质量不稳定的弊端。
2.也不需要乙醇沉淀等容易丧失微小分子RNA的步骤。
3.快速,简捷,单个样品操作一般可在30分钟内完成。
4.多次柱漂洗确保高纯度,OD260/OD280典型的比值达1.9~2.0,基本无DNA残留,可用于RNAi,RT-PCR,Northern-blot和各种实验。

 The microRNA Kit combines phenol/guanidine-based lysis of samples and silicamembrane based purification of total RNA. Lysis/Binding buffer is a monophasic solution of phenol and guanidine thiocyanate, designed to facilitate lysis of tissues, to inhibit RNases, and also to remove most of the cellular DNA and proteins from the lysate by organic extraction. 
    Cells or tissue samples are homogenized in Lysis/Binding buffer. After addition of chloroform, the homogenate is separated into aqueous and organic phases by centrifugation. RNA partitions to the upper, aqueous phase, while DNA partitions to the interphase and proteins to the lower, organic phase or the interphase. 
     The upper, aqueous phase is extracted, and ethanol is added to provide appropriate binding conditions for all RNA molecules from 18 nucleotides (nt) upwards. The sample is then applied to the RNA Bind columns, where the total RNA binds to the membrane and phenol and other contaminants are efficiently washed away. Highquality RNA is then eluted in RNase-free water. 
    For enrichment of miRNAs and other small RNAs (less than ~200 nt) in a separate fraction, a specialized protocol is provided in Appendix A, page 31. Enrichment of small RNAs in a separate fraction may be advantageous for certain applications where mRNA and rRNA could lead to increased background. For this specialized protocol, an additional microRNA Bind Columns is inclued.