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组织/细胞基因组DNA快速提取试剂盒

1407-组织 细胞基因组DNA快速提取试剂盒
目录号:1407
目录编号 包装单位
140701 50
140702 100
140703 200
140711 50(蛋白酶K粉)
140712 100次(带蛋白酶K粉)
140713 200次(带蛋白酶K粉)

适用范围:
适用于快速提取各种动植物细胞/组织基因组DNA

产品介绍:
独特的结合液/蛋白酶K迅速裂解细胞和灭活细胞内核酸酶,然后基因组DNA在高离序盐状态下选择性吸附于离心柱内硅基质膜, 再通过一系列快速的漂洗-离心的步骤, 抑制物去除液和漂洗液将细胞代谢物、蛋白等杂质去除, 最后低盐的洗脱缓冲液将纯净基因组DNA从硅基质膜上洗脱。
 
产品特点:
1.离心吸附柱内硅基质膜全部采用进口世界著名公司特制吸附膜,柱与柱之间吸附量差异极小,可重复性好。克服了国产试剂盒膜质量不稳定的弊端。
2.不需要使用有毒的苯酚等试剂,也不需要乙醇沉淀等步骤。
3.快速,简捷,单个样品操作一般可在30分钟内完成。
4.多次柱漂洗确保高纯度,OD260/OD280典型的比值达1.7~1.9,长度可达30kb -50kb,可直接用于PCR,Southern-blot和各种酶切反应。

Cells are lysed during a short incubation with Proteinase K in the presence of a chaotropic salt (guanidine-HCl), which immediately inactivates all nucleases. Cellular nucleic acids (NA) bind selectively to special silicon membrane pre-packed in the spin columns. Bound NA is purified in a series of rapid "wash-and-spin" steps to remove contaminating cellular components. A special Inhibitor Removal Buffer (Buffer IR) has been included which allows even the application of heparinized sample material with 100 U/ml of Heparin. Finally, low salt elution releases the NA from the silicon membrane. This simple method eliminates the need for organic solvent extractions and DNA precipitation, allowing for rapid purification of many samples simultaneously.