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Fast研磨法土壤基因组DNA提取试剂盒

目录号:1445
目录编号  包装单位
144501     10次

Fast研磨法土壤基因组DNA提取试剂盒结合了两家公司产量高和纯度高的优点研制而成,采取(3种研磨珠混合)的研磨管方案可以处理更大的样品,可以直接使用一样的研磨仪和参数。独有的去腐殖酸技术可以完美去除各种腐殖酸和杂质的影响,提取的DNA纯度高,可以直接用于下游PCR反应。


Description

The Fast Soil DNA Kit quickly and efficiently isolates PCR-ready genomic DNAdirectly from soil samples in less than 40 minutes. Designed for use with Beads-Beating device such as the FastPrep® Instruments from MP Biomedicalssoil organisms population are easily lysed within 40 seconds. Samples are placed into 2.0 ml tubes containing 3 kinds of beads, a mixture of ceramic and silica particles designed to efficiently lyse all soil organisms including historically difficult sources such as eubacterial spores and endospores, gram positive bacteria, yeast, algae, nematodes and fungi.

The kit uses a novel and proprietary method to remove high humic acid content including difficult soil types such as compost, sediment, and manure.The isolated DNA has a high level of purity allowing for more successful PCR amplification of organisms from the sample. PCR analysis has been performed to detect a variety of organisms including bacteria (e.g. Bacillus subtilis, Bacillus anthracis), fungi (e.g. yeasts, molds), algae and Actinomycetes (e.g. Streptomyces).
 

Important consideration before use

The fill volume in the bead tube after the addition of the Sodium Phosphate and MT Buffers to the sample should allow sufficient air space in the sample tube for efficient FastPrep® Instrument processing. MP Biomedicals recommends using 500 mg of starting material as long as there is between 250 - 500 μl of empty space in the tube. Sample loss or tube failure may result from overfilling the bead tube. The bead tube caps must be secure, but not over-tightened, to prevent sample leakage. If the sample is too large for processing in a single tube, divide the sample and process using multiple tubes. The kits have been rigorously tested in the FastPrep® Instrument. A single 40 second run at a speed setting of 6.0 in the FastPrep® Instrument is sufficient to lyse almost all samples. If the user experimentally determines that additional processing time is required, the sample should be incubated on ice in the bead tube for at least 2 minutes between successive FastPrep® Instrument homogenizations to prevent overheating the sample and tube.   

 

If you use other bead beater device, please follow instruction manual from manufaturer to set appropriate parameter for good performance.