蛋白酶K  Proteinase K

规格：100mg

储存：干粉状态可在0~4℃条件下低温保藏。溶解后分装为适当体积，-20oC贮存。

蛋白酶K是一 种非特异性蛋白内切酶，该酶属丝氨酸类蛋白酶，可以切割脂肪族氨基酸、疏水性氨基酸和芳香族氨基酸羧基端连接的酯键和肽键。分子量大小为28.9 kDa（单体），在pH4到pH12的条件下均有活性，在SDS、尿素或EDTA存在时亦很稳定。它能够切割脂族氨基酸和芳香族氨基酸的羧基端肽键，用于 生物样品中蛋白质的降解。此酶经脱色和色谱纯化去除了RNA和DNA，也检测不到其它杂酶活性。由于蛋白酶K在尿素和SDS中稳定，还具有降解天然蛋白质 的能力，其水解的最小多肽是四肽分子。

Description:

     Proteinase K is a broad substrate non-specific serine proteinase. It is very stable at pH 4-12. It was used on isolating mRNA, genomic DNA and digesting unwanted proteins during DNA and RNA preparations from different kinds of cells. It’s been used on glycoprotein modification and protein structure studies also. Proteinase K is active with SDS, urea and EDTA. 

Store:

       Store vial at 2-4ºC upon receipt. Dissolve and aliquot and store at -20ºC Recommended.
      Expiration date is one year of powder and six months of sterile liquid at -20ºC to 4ºC. Use sterile   filtration or add 0.5% NaN3 for liquid enzyme storage if keep it at 2-8ºC over a week.